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KMID : 0613820100200081201
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Journal of Life Science
2010 Volume.20 No. 8 p.1201 ~ p.1206
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Comparative Analysis of Microcystin during Water Treatment Process between Real-Time PCR and LC/MS
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Park Hong-Gi
Jung Mi-Eun
Cha Dong-Jin
Jung Eun-Young
Bean Jae-Hoon
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Abstract
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We performed a comparative analysis using a Real-time PCR (Polymerase Chain Reaction) and LC/MS (Liquid-Chromatograph/Mass Spectrometer) method in order to detect microcystin in environmental sources. Among the three different primer sets tested for microcystin using three positive strains of Microcystis aeruginosa by Real-time PCR assay, only TOX2P/TOX2M primer pairs were able to detect Microcystis aeruginosa. According to the results of a survey carried out from June 2009 to September 2009, 11 out of 11 (100%) raw water samples were were found to have microcystin when the Real-Time PCR and LC/MS method was used, with total microcystin concentration ranging from 5.98~219.0 ㎍/l. A microcystin removal treatment process was used to ensure entire removal, by passing it through a BAC filtration step. It was concluded that real-time PCR assay can be used to estimate micrucystin detection more rapidly and easily than the LC/MS method.
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KEYWORD
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Microcystin, Real-Time PCR, LC/MS, TOX2P/TOX2M primer
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